Antihepatic Fibrosis Effect of Active Components Isolated from Green Asparagus (Asparagus officinalis L.) Involves the Inactivation of Hepatic Stellate Cells

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• 作者：Chunge Zhong ; Chunyu Jiang ; Xichun Xia ; Teng Mu ; Lige Wei ; Yuntian Lou ; Xiaoshu Zhang ; Yuqing Zhao ; Xiuli Bi
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：July 8, 2015
• 年：2015
• 卷：63
• 期：26
• 页码：6027-6034
• 全文大小：463K
• ISSN：1520-5118

文摘

Green asparagus (Asparagus officinalis L.) is a vegetable with numerous nutritional properties. In the current study, a total of 23 compounds were isolated from green asparagus, and 9 of these compounds were obtained from this genus for the first time. Preliminary data showed that the ethyl acetate (EtOAc)-extracted fraction of green asparagus exerted a stronger inhibitory effect on the growth of t-HSC/Cl-6 cells, giving an IC₅₀ value of 45.52 渭g/mL. The biological activities of the different compounds isolated from the EtOAc-extracted fraction with respect to antihepatic fibrosis were investigated further. Four compounds, C3, C4, C10, and C12, exhibited profound inhibitory effect on the activation of t-HSC/Cl-6 cells induced by TNF-伪. The activation t-HSC/Cl-6 cells, which led to the production of fibrotic matrix (TGF-尾1, activin C) and accumulation of TNF-伪, was dramatically decreased by these compounds. The mechanisms by which these compounds inhibited the activation of hepatic stellate cells appeared to be associated with the inactivation of TGF-尾1/Smad signaling and c-Jun N-terminal kinases, as well as the ERK phosphorylation cascade.