Preparation and Characterization of Dictyophora indusiata Polysaccharide鈥揨inc Complex and Its Augmented Antiproliferative Activity on Human Cancer Cells

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• 作者：Wenzhen Liao ; Yunjun Lu ; Junning Fu ; Zhengxiang Ning ; Jiguo Yang ; Jiaoyan Ren
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：July 29, 2015
• 年：2015
• 卷：63
• 期：29
• 页码：6525-6534
• 全文大小：604K
• ISSN：1520-5118

文摘

Dictyophora indusiata polysaccharide (DP1) was successfully chelated with zinc chloride to achieve its enhanced antiproliferative activity. The obtained DP1鈥揨n complex showed significant antiproliferative activity (18.1 卤 2.84% viability of MCF-7 cells at 250 渭g/mL) on a group of human cancer cell lines through induction of apoptosis. The pro-apoptotic action of DP1鈥揨n was confirmed by morphological changes including chromatin condensation, DNA breakage, and S phase cell cycle arrest in human breast adenocarcinoma cells (MCF-7). The DP1鈥揨n-induced apoptotic pathways were characterized by the activation of caspases-3, -8, and -9, mitochondrial dysfunction, and reactive oxygen species (ROS) overproduction (305 卤 7.06% production of control at 250 渭g/mL). This study suggested that DP1鈥揨n can be developed as a candidate for cancer treatment and prevention, especially human breast adenocarcinoma.