Molecular Characterization of Polycyclic Aromatic Hydrocarbon (PAH)-Degrading Methanogenic Communities

详细信息    查看全文

• 作者：Wook Chang ; Youngsoon Um ; Brendan Hoffman ; and Tracey R. Pulliam Holoman
• 刊名：Biotechnology Progress
• 出版年：2005
• 出版时间：June 2005
• 年：2005
• 卷：21
• 期：3
• 页码：682 - 688
• 全文大小：295K
• 年卷期：v.21,no.3(June 2005)
• ISSN：1520-6033

文摘

Previous research demonstrated that methanogenic cultures enriched from BaltimoreHarbor (Baltimore, MD) sediments were able to degrade naphthalene and phenanthrene. In this report, the degradation activity was maintained through a sequentialtransfer without adding additional sediments and the established polycyclic aromatichydrocarbon (PAH)-degrading methanogenic communities were characterized viacomparative sequence analysis of clone libraries of 16S rRNA genes amplified usingbacteria-specific and Archaea-specific primers. The phylogenetic analysis indicatedthat the addition of PAHs clearly shifted the structure of the methanogenic communityand resulted in an increase in populations of species previously found in otherhydrocarbon-degrading communities. Of particular interest is the fact that thedominant microbial population of the naphthalene cultures was different from that ofthe phenanthrene cultures, suggesting that different species are involved in thedegradation. Finally, this information may lead to the identification and isolation ofmethanogenic populations that can degrade PAHs.