Metabolomic Characterization of Malolactic Fermentation and Fermentative Behaviors of Wine Yeasts in Grape Wine

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• 作者：Hong-Seok Son ; Geum-Sook Hwang ; Won-Mok Park ; Young-Shick Hong ; Cherl-Ho Lee
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2009
• 出版时间：June 10, 2009
• 年：2009
• 卷：57
• 期：11
• 页码：4801-4809
• 全文大小：1042K
• 年卷期：v.57,no.11(June 10, 2009)
• ISSN：1520-5118

文摘

Wine contains a number of metabolites that are produced during alcoholic and malolactic fermentations (MLF) or aging, which are important compounds for determining wine quality. This study investigated changes in metabolites in wines to characterize malolactic fermentation (MLF) and to assess fermentative behaviors of wine yeast strains using ¹H nuclear magnetic resonance (NMR) spectroscopy coupled with multivariate statistics. Principal component analysis (PCA) showed clear differentiation between non- and induced-malolactic fermented wines by wine lactic acid bacteria (LAB) and between wines fermented with various wine yeast strains. Metabolites such as glycerol, lactate, 2,3-butanediol, succinate, leucine, isoleucine, alanine, valine, proline, choline, γ-aminobutyric acid (GABA), and polyphenols contributed to the differentiations. Decreased levels of malate and citrate along with increased levels of lactate were the metabolites most responsible for the differentiation of induced-MLF wines from non-MLF wines. In particular, high succinate levels provided evidence of an inhibitory effect of Saccharomyces bayanus against spontaneous MLF. Furthermore, dependence of metabolites on wine yeast strains was observed, demonstrating their different fermentative behaviors. This study demonstrates that wine fermentation by yeast and LAB can be characterized through global and multivariate statistical analysis of ¹H NMR spectral data.