Gold Nanoclusters@Ru(bpy)32+-Layered Double Hydroxide Ultrathin Film as a Cathodic Electrochemiluminescence Resonance Energy Transfer Probe
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- 作者：Yingchang Yu ; Chao Lu ; Meining Zhang
- 刊名：Analytical Chemistry
- 出版年：2015
- 出版时间：August 4, 2015
- 年：2015
- 卷：87
- 期：15
- 页码：8026-8032
- 全文大小：352K
- ISSN：1520-6882
文摘
Herein, it is the first report that a cathodic electrochemiluminescence (ECL) resonance energy transfer (ERET) system is fabricated by layer-by-layer (LBL) electrostatic assembly of CoAl layered double hydroxide (LDH) nanosheets with a mixture of blue BSA鈥揼old nanoclusters (AuNCs) and Ru(bpy)32+ (denoted as AuNCs@Ru) on an Au electrode. The possible ECL mechanism indicates that the appearance of CoAl鈥揕DH nanosheets generates a long-range stacking order of the AuNCs@Ru on an Au electrode, facilitating the occurrence of the ERET between BSA鈥揂uNC donors and Ru(bpy)32+ acceptors on the as-prepared AuNCs@Ru鈥揕DH ultrathin films (UTFs). Furthermore, it is observed that the cathodic ECL intensity can be quenched efficiently in the presence of 6-mercaptopurine (6-MP) in a linear range of 2.5鈥?00 nM with a detection limit of 1.0 nM. On the basis of these interesting phenomena, a facile cathodic ECL sensor has successfully distinguished 6-MP from other thiol-containing compounds (e.g., cysteine and glutathione) in human serum and urine samples. The proposed sensing scheme opens a way for employing the layered UTFs as a platform for the cathodic ECL of Ru(bpy)32+.