A Dual Platform for Selective Analyte Enrichment and Ionization in Mass Spectrometry Using Aptamer-Conjugated Graphene Oxide

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• 作者：Basri Gulbakan ; Emir Yasun ; M. Ibrahim Shukoor ; Zhi Zhu ; Mingxu You ; Xiaohong Tan ; Hernan Sanchez ; David H. Powell ; Hongjie Dai ; Weihong Tan
• 刊名：Journal of the American Chemical Society
• 出版年：2010
• 出版时间：December 15, 2010
• 年：2010
• 卷：132
• 期：49
• 页码：17408-17410
• 全文大小：153K
• 年卷期：v.132,no.49(December 15, 2010)
• ISSN：1520-5126

文摘

This study demonstrates the use of aptamer-conjugated graphene oxide as an affinity extraction and detection platform for analytes from complex biological media. We have shown that cocaine and adenosine can be selectively enriched from plasma samples and that direct mass spectrometric readouts can be obtained without a matrix and with greatly improved signal-to-noise ratios. Aptamer-conjugated graphene oxide has clear advantages in target enrichment and in generating highly efficient ionization of target molecules for mass spectrometry. These results demonstrate the utility of the approach for analysis of small molecules in real biological samples.