Visible Study of Mercuric Ion and Its Conjugate in Living Cells of Mammals and Plants

详细信息    查看全文

• 作者：Zhichao Zhang ; Di Wu ; Xiangfeng Guo ; Xuhong Qian ; Zhe Lu ; Qin Xu ; Yuanyuan Yang ; Liping Duan ; Yuke He ; and Zhe Feng
• 刊名：Chemical Research in Toxicology
• 出版年：2005
• 出版时间：December 2005
• 年：2005
• 卷：18
• 期：12
• 页码：1814 - 1820
• 全文大小：360K
• 年卷期：v.18,no.12(December 2005)
• ISSN：1520-5010

文摘

The investigation of mercuric ion and mercuric conjugate inside live specimens has drawnintense attention because of their cytotoxicity. The translocation, transportation, and distribution of Hg²⁺ inside either mammals or plants, however, are still invisible due to the lack offavorable fluorescent molecular sensors for Hg²⁺. Here, two sensors, 2,6-bis(4'-peperazino-N'-hydroxylethoxylethylene-1',8'-naphthalimide)dimethylpyridine and 2,6-bis(4'-peperazino-N'-butyl-1',8'-naphthalimide)dimethylpyridine, which were composed of nitrogen atoms of 2,6-bis(aminomethyl)pyridine as the ion receptor and the donor of photoinduced electron transfer,were applied to the cultured mammalian cells and plant cells. Their membrane permeability,low toxicity, slow bleaching/fading, and high selectivity and sensitivity to Hg²⁺ in a live cellrendered them attractive to become real-time and real-space sensors. For the first time, thetransportation of Hg²⁺ cation and Hg²⁺ conjugate of cysteine was observed with the help of afluorescence microscope. The chloroplast location of Hg²⁺ in transgenic tobacco was also visible.