Chemical Fluorescent Probe for Detection of A尾 Oligomers
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- 作者：Chai Lean Teoh ; Dongdong Su ; Srikanta Sahu ; Seong-Wook Yun ; Eleanor Drummond ; Frances Prelli ; Sulgi Lim ; Sunhee Cho ; Sihyun Ham ; Thomas Wisniewski ; Young-Tae Chang
- 刊名：Journal of the American Chemical Society
- 出版年：2015
- 出版时间：October 28, 2015
- 年：2015
- 卷：137
- 期：42
- 页码：13503-13509
- 全文大小：389K
- ISSN：1520-5126
文摘
Aggregation of amyloid 尾-peptide (A尾) is implicated in the pathology of Alzheimer鈥檚 disease (AD), with the soluble, A尾 oligomeric species thought to be the critical pathological species. Identification and characterization of intermediate species formed during the aggregation process is crucial to the understanding of the mechanisms by which oligomeric species mediate neuronal toxicity and following disease progression. Probing these species proved to be extremely challenging, as evident by the lack of reliable sensors, due to their heterogeneous and transient nature. We describe here an oligomer-specific fluorescent chemical probe, BoDipy-Oligomer (BD-Oligo), developed through the use of the diversity-oriented fluorescent library approach (DOFLA) and high-content, imaging-based screening. This probe enables dynamic oligomer monitoring during fibrillogenesis in vitro and shows in vivo A尾 oligomers staining possibility in the AD mice model.