Suspended Microchannel Resonators for Ultralow Volume Universal Detection

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• 作者：Sungmin Son ; William H. Grover ; Thomas P. Burg ; Scott R. Manalis
• 刊名：Analytical Chemistry
• 出版年：2008
• 出版时间：June 15, 2008
• 年：2008
• 卷：80
• 期：12
• 页码：4757 - 4760
• 全文大小：355K
• 年卷期：v.80,no.12(June 15, 2008)
• ISSN：1520-6882

文摘

Universal detectors that maintain high sensitivity as the detection volume is reduced to the subnanoliter scale can enhance the utility of miniaturized total analysis systems (μ-TAS). Here the unique scaling properties of the suspended microchannel resonator (SMR) are exploited to show universal detection in a 10 pL analysis volume with a density detection limit of ~1 µg/cm³ (10 Hz bandwidth) and a dynamic range of 6 decades. Analytes with low UV extinction coefficients such as polyethylene glycol (PEG) 8 kDa, glucose, and glycine are measured with molar detection limits of 0.66, 13.5, and 31.6 µM, respectively. To demonstrate the potential for real-time monitoring, gel filtration chromatography was used to separate different molecular weights of PEG as the SMR acquired a chromatogram by measuring the eluate density. This work suggests that the SMR could offer a simple and sensitive universal detector for various separation systems from liquid chromatography to capillary electrophoresis. Moreover, since the SMR is itself a microfluidic channel, it can be directly integrated into μ-TAS without compromising overall performance.