Microfluidic Serial Dilution Circuit

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• 作者：Brian M. Paegel ; William H. Grover ; Alison M. Skelley ; Richard A. Mathies ; Gerald F. Joyce
• 刊名：Analytical Chemistry
• 出版年：2006
• 出版时间：November 1, 2006
• 年：2006
• 卷：78
• 期：21
• 页码：7522 - 7527
• 全文大小：275K
• 年卷期：v.78,no.21(November 1, 2006)
• ISSN：1520-6882

文摘

In vitro evolution of RNA molecules requires a methodfor executing many consecutive serial dilutions. To solvethis problem, a microfluidic circuit has been fabricatedin a three-layer glass-PDMS-glass device. The 400-nLserial dilution circuit contains five integrated membranevalves: three two-way valves arranged in a loop to drivecyclic mixing of the diluent and carryover, and two busvalves to control fluidic access to the circuit through inputand output channels. By varying the valve placement inthe circuit, carryover fractions from 0.04 to 0.2 wereobtained. Each dilution process, which is composed of adiluent flush cycle followed by a mixing cycle, is carriedout with no pipeting, and a sample volume of 400 nL issufficient for conducting an arbitrary number of serialdilutions. Mixing is precisely controlled by changing thecyclic pumping rate, with a minimum mixing time of 22 s.This microfluidic circuit is generally applicable for integrating automated serial dilution and sample preparationin almost any microfluidic architecture.