Identification of Penicillin G Metabolites under Various Environmental Conditions Using UHPLC-MS/MS

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• 作者：Fadi Aldeek ; Daniele Canzani ; Matthew Standland ; Mark R. Crosswhite ; Walter Hammack ; Ghislain Gerard ; Jo-Marie Cook
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：August 10, 2016
• 年：2016
• 卷：64
• 期：31
• 页码：6100-6107
• 全文大小：623K
• 年卷期：0
• ISSN：1520-5118

文摘

In this work, we investigate the stability of penicillin G in various conditions including acidic, alkaline, natural acidic matrices and after treatment of citrus trees that are infected with citrus greening disease. The identification, confirmation, and quantitation of penicillin G and its various metabolites were evaluated using two UHPLC-MS/MS systems with variable capabilities (i.e., Thermo Q Exactive Orbitrap and Sciex 6500 QTrap). Our data show that under acidic and alkaline conditions, penicillin G at 100 ng/mL degrades quickly, with a determined half-life time of approximately 2 h. Penillic acid, penicilloic acid, and penilloic acid are found to be the most abundant metabolites of penicillin G. These major metabolites, along with isopenillic acid, are found when penicillin G is used for treatment of citrus greening infected trees. The findings of this study will provide insight regarding penicillin G residues in agricultural and biological applications.