Comparison of Duplex Stabilizing Properties of 2鈥?Fluorinated Nucleic Acid Analogues with Furanose and Non-Furanose Sugar Rings
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- 作者：Michael E. 脴stergaard ; Timothy Dwight ; Andres Berdeja ; Eric E. Swayze ; Michael E. Jung ; Punit P. Seth
- 刊名：Journal of Organic Chemistry
- 出版年：2014
- 出版时间：September 19, 2014
- 年：2014
- 卷：79
- 期：18
- 页码：8877-8881
- 全文大小：344K
- ISSN：1520-6904
文摘
We compare the duplex stabilizing properties of 2鈥?fluorinated nucleic acid analogues with furanose and non-furanose ring systems and dissect the relative contributions of hydration, sugar conformation, and fluorine configuration toward the overall Tm value. We find that the stabilization imparted by fluorine substitution is additive over that obtained by restricting the conformation of the sugar ring itself. Our studies support further evaluation of fluorinated nucleic acid analogues with non-furanose sugar rings as surrogates of 2鈥?F RNA for therapeutic antisense applications.