Toward a Selective, Sensitive, Fast-Responsive, and Biocompatible Two-Photon Probe for Hydrogen Sulfide in Live Cells

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• 作者：Subhankar Singha ; Dokyoung Kim ; Hyunsoo Moon ; Taejun Wang ; Ki Hean Kim ; Youn Ho Shin ; Junyang Jung ; Eunseok Seo ; Sang-Joon Lee ; Kyo Han Ahn
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：January 20, 2015
• 年：2015
• 卷：87
• 期：2
• 页码：1188-1195
• 全文大小：451K
• ISSN：1520-6882

文摘

Hydrogen sulfide has emerged as an exciting endogenous gasotransmitter in addition to nitric oxide and carbon dioxide. Noninvasive detection methods for hydrogen sulfide thus become indispensable tools for studying its diverse roles in biological systems. Accordingly, fluorescent probes for hydrogen sulfide have received great attention in recent years. A practically useful fluorescent probe for bioimaging of hydrogen sulfide should be selective, sensitive, fast-responsive, biocompatible, observable in the biological optical window, and capable of deep-tissue imaging. These sensing properties, however, are extremely difficult to achieve at the same time. Disclosed here is the two-photon fluorescent probe that meets all of these criteria. The probe belongs to a Michael acceptor system, which raised a serious selectivity issue over the competing biothiols such as cysteine and glutathione. We have addressed the selectivity issue by optimizing the electronic and steric interactions between biothiols and the probe, in addition to achieving very high sensitivity, fast-response, and biocompatibility. Also, the sensing mechanism suggested in the literature was revised. The probe thus enables us to image the endogenously produced hydrogen sulfide with negligible interference from other biothiols in live cells. The excellent sensing properties of the probe combined with its capability of bioimaging thus make it a practically useful tool for further studying biological roles of hydrogen sulfide.