Determination of N2-Hydroxymethyl-dG Adducts in the Nasal Epithelium and Bone Marrow of Nonhuman Primates Following 13CD2-Formaldehyde Inhalation Exposur

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• 作者：Benjamin C. Moeller ; Kun Lu ; Melanie Doyle-Eisele ; Jacob McDonald ; Andrew Gigliotti ; James A. Swenberg
• 刊名：Chemical Research in Toxicology
• 出版年：2011
• 出版时间：February 18, 2011
• 年：2011
• 卷：24
• 期：2
• 页码：162-164
• 全文大小：697K
• 年卷期：v.24,no.2(February 18, 2011)
• ISSN：1520-5010

文摘

The presence of endogenous and exogenous N²-hydroxymethyl-dG adducts in DNA from the nasal mucosa and bone marrow of cynomolgus macaques exposed to 1.9 and 6.1 ppm of [¹³CD₂]-formaldehyde for 6 h a day for 2 consecutive days was investigated using a highly sensitive nano-UPLC-MS/MS method with a limit of detection of 20 amol. Both exogenous and endogenous adducts were readily detected and quantified in the nasal tissues of both exposure groups, with an exposure dependent increase in exogenous adducts observed. In contrast, only endogenous adducts were detectable in the bone marrow, even though 10 times more DNA was analyzed.