Flow Injection Analysis System Equipped with a Newly Designed Electrochemiluminescent Detector and Its Application for Detection of 2-Thiouracil

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• 作者：Yuwu Chi ; Jianping Duan ; Shudan Lin ; and Guonan Chen
• 刊名：Analytical Chemistry
• 出版年：2006
• 出版时间：March 1, 2006
• 年：2006
• 卷：78
• 期：5
• 页码：1568 - 1573
• 全文大小：310K
• 年卷期：v.78,no.5(March 1, 2006)
• ISSN：1520-6882

文摘

A new flow injection analysis (FIA) system equipped withan electrochemiluminescent (ECL) detector has beendeveloped and applied for the ECL detection of 2-thiouracil. The FIA-ECL system used a specially designed flow-through ECL thin-layer cell to reduce the dead volume,the IR drop across the cell, and the probability ofaccumulation of gas bubbles in the cell. It was thusenvisioned to improve the detection limit of the FIA-ECLmethod. After being established, the new FIA-ECL systemwas used to investigate the ECL response of 2-thiouracilin the presence of the ECL of Ru(bpy)₃²⁺. It was foundthat 2-thiouracil could enhance the ECL of Ru(bpy)₃²⁺over a wide pH range (pH 4.0-12.0). A highly sensitivemethod for detection of 2-thiouracil in biological sampleswas developed by the new FIA-ECL system after optimizing several experimental conditions, such as the appliedpotential of the working electrode, the pH value of theaqueous solution, the flow rate of carrier solution, andthe concentration of Ru(bpy)₃²⁺.