Non-Ionic Amphiphilic Homopolymers: Synthesis, Solution Properties, and Biochemical Validation

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• 作者：K. Shivaji Sharma ; Gr茅gory Durand ; Frank Gabel ; Paola Bazzacco ; Christel Le Bon ; Emmanuelle Billon-Denis ; Laurent J. Catoire ; Jean-Luc Popot ; Christine Ebel ; Bernard Pucci
• 刊名：Langmuir
• 出版年：2012
• 出版时间：March 13, 2012
• 年：2012
• 卷：28
• 期：10
• 页码：4625-4639
• 全文大小：718K
• 年卷期：v.28,no.10(March 13, 2012)
• ISSN：1520-5827

文摘

A novel type of nonionic amphipols for handling membrane proteins in detergent-free aqueous solutions has been obtained through free-radical homo-telomerization of an acrylamide-based monomer comprising a C₁₁ alkyl chain and two glucose moieties, using a thiol as transfer reagent. By controlling the thiol/monomer ratio, the number-average molecular weight of the polymers was varied from 8 to 63 kDa. Homopolymeric nonionic amphipols were found to be highly soluble in water and to self-organize, within a large concentration range, into small, compact particles of 6 nm diameter with a narrow size distribution, regardless of the molecular weight of the polymer. They proved able to trap and stabilize two test membrane proteins, bacteriorhodopsin from Halobium salinarum and the outer membrane protein X of Escherichia coli, under the form of small and well-defined complexes, whose size, composition, and shape were studied by aqueous size-exclusion chromatography, analytical ultracentrifugation, and small-angle neutron scattering. As shown in a companion paper, nonionic amphipols can be used for membrane protein folding, cell-free synthesis, and solution NMR studies (Bazzacco et al. 2012, Biochemistry, DOI: 10.1021/bi201862v).