Aggregation Control of Squaraines and Their Use as Near-Infrared Fluorescent Sensors for Protein

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• 作者：Yongqian Xu ; Zhiyong Li ; Andrey Malkovskiy ; Shiguo Sun ; Yi Pang
• 刊名：Journal of Physical Chemistry B
• 出版年：2010
• 出版时间：July 1, 2010
• 年：2010
• 卷：114
• 期：25
• 页码：8574-8580
• 全文大小：430K
• 年卷期：v.114,no.25(July 1, 2010)
• ISSN：1520-5207

文摘

A series of squaraine dyes are found to be in H- or J-aggregates and are almost nonfluorescent in aqueous solution. Upon addition of bovine serum albumin (BSA), however, the fluorescence intensity (at λ_em ≈ 690 nm) increases by a factor of up to 200. Transformation of the dye molecules from the aggregates to the monomeric species appears to be responsible for the large fluorescence turn-on. While both H- and J-aggregates contribute to the observed fluorescence turn-on, the former appears to play a more important role. Electrophoresis imaging shows that these probes are good BSA indicators.