Identification of N-Acylphosphatidylserine Molecules in Eukaryotic Cells

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• 作者：Ziqiang Guan ; Shengrong Li ; Dale C. Smith ; Walter A. Shaw ; Christian R. H. Raetz
• 刊名：Biochemistry
• 出版年：2007
• 出版时间：December 18, 2007
• 年：2007
• 卷：46
• 期：50
• 页码：14500 - 14513
• 全文大小：409K
• 年卷期：v.46,no.50(December 18, 2007)
• ISSN：1520-4995

文摘

While profiling the lipidome of the mouse brain by mass spectrometry, we discovered a novelfamily of N-acylphosphatidylserine (N-acyl-PS) molecules. These N-acyl-PS species were enriched byDEAE-cellulose column chromatography, and they were then characterized by accurate mass measurements,tandem mass spectrometry, liquid chromatography/mass spectrometry, and comparison to an authenticstandard. Mouse brain N-acyl-PS molecules are heterogeneous and constitute about 0.1% of the totallipid. In addition to various ester-linked fatty acyl chains on their glycerol backbones, the complexity ofthe N-acyl-PS series is further increased by the presence of diverse amide-linked N-acyl chains, whichinclude saturated, monounsaturated, and polyunsaturated species. N-Acyl-PS molecular species were alsodetected in the lipids of pig brain, mouse RAW264.7 macrophage tumor cells, and yeast, but not Escherichiacoli. N-Acyl-PSs may be biosynthetic precursors of N-acylserine molecules, such as the recently reportedsignaling lipid N-arachidonoylserine from bovine brain. We suggest that a phospholipase D might cleaveN-acyl-PS to generate N-acylserine, in analogy to the biosynthesis of the endocannabinoid N-arachidonoylethanolamine (anadamide) from N-arachidonoylphosphatidylethanolamine.