Fluorescence Light-Up Probe for Parallel G-Quadruplexes

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• 作者：Bing Jin ; Xin Zhang ; Wei Zheng ; Xiangjun Liu ; Cui Qi ; Fuyi Wang ; Dihua Shangguan
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：January 7, 2014
• 年：2014
• 卷：86
• 期：1
• 页码：943-952
• 全文大小：528K
• ISSN：1520-6882

文摘

Putative G-quadruplex-forming sequences (PQS) are highly prevalent in human genome; however, the structures and functions of most PQSs in genome are poorly understood. Therefore, selective recognition of certain types of G-quadruplexes (G4s) is important for the study of G4s. A new light up fluorescent probe, BPBC composed of benzimidazole and carbazole moieties was designed and synthesized. BPBC possesses a crescent-shaped 蟺-conjugated planar core that is slightly larger than the dimension of the G-quartet plane in G4s. This structure endows BPBC with excellent selectivity to parallel G4s. BPBC exhibits almost no fluorescence in the aqueous buffer condition, its fluorescence increases approximately 330鈥?800-fold in the presence of parallel G4s but only about 30-fold in the presence of single/double-stranded (ss/ds) DNA and 30鈥?10-fold in the presence of antiparallel G4s. Binding studies indicate that the highly selective fluorescent response of BPBC arises from end-stack binding model to G-quartet.