Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites

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• 作者：Christopher T. Veldkamp ; Eva Kiermaier ; Skylar J. Gabel-Eissens ; Miranda L. Gillitzer ; David R. Lippner ; Frank A. DiSilvio ; Casey J. Mueller ; Paeton L. Wantuch ; Gary R. Chaffee ; Michael W. Famiglietti ; Danielle M. Zgoba ; Asha A. Bailey ; Yaya Bah ; Samantha J. Engebretson ; David R. Graupner ; Emily R. Lackner ; Vincent D. LaRosa ; Tysha Medeiros ; Michael L. Olson ; Andrew J. Phillips ; Harley Pyles ; Amanda M. Richard ; Scott J. Schoeller ; Boris Touzeau ; Larry G. Williams ; Michael Sixt ; Francis C. Peterson
• 刊名：Biochemistry
• 出版年：2015
• 出版时间：July 14, 2015
• 年：2015
• 卷：54
• 期：27
• 页码：4163-4166
• 全文大小：202K
• ISSN：1520-4995

文摘

CCL19 and CCL21 are chemokines involved in the trafficking of immune cells, particularly within the lymphatic system, through activation of CCR7. Concurrent expression of PSGL-1 and CCR7 in naive T-cells enhances recruitment of these cells to secondary lymphoid organs by CCL19 and CCL21. Here the solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is competitive. Implications for the mechanism of PSGL-1鈥檚 enhancement of resting T-cell recruitment are discussed.