Polypyrrole-Entrapped Quinohemoprotein Alcohol Dehydrogenase. Evidence for Direct Electron Transfer via Conducting-Polymer Chains
详细信息 查看全文
- 作者：Arunas Ramanavicius ; Katja Habermü ; ller ; Elisabeth Csö ; regi ; Valdas Laurinavicius ; and Wolfgang Schuhmann
- 刊名：Analytical Chemistry
- 出版年：1999
- 出版时间：August 15, 1999
- 年：1999
- 卷：71
- 期：16
- 页码：3581 - 3586
- 全文大小：89K
- 年卷期：v.71,no.16(August 15, 1999)
- ISSN：1520-6882
文摘
It is reported for the first time that direct electron-transferprocesses between a polypyrrole (PPY) entrapped quinohemoprotein alcohol dehydrogenase from Gluconobactersp. 33 (QH-ADH) and a platinum electrode take place viathe conducting-polymer network. The cooperative actionof the enzyme-integrated prosthetic groups-pyrroloquinoline-quinone and hemes-is assumed to allow this electron-transfer pathway from the enzyme's active site to theconducting-polymer backbone. A hypothetical model ofthe electron transfer is proposed which is supported bythe influence of various parameters, such as, e.g., ionicstrength and nature of the buffer salts. This unusualelectron-transfer pathway leads to an accentuated increaseof the KMapp value (102 mM) and hence to a significantlyincreased linear detection range of an ethanol sensorbased on this enzyme.