Interaction of Tissue Engineering Substrates with Serum Proteins and Its Influence on Human Primary Endothelial Cells

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• 作者：Tamilselvan Mohan ; Katrin Niegelhell ; Chandran Nagaraj ; David Reishofer ; Stefan Spirk ; Andrea Olschewski ; Karin Stana Kleinschek ; Rupert Kargl
• 刊名：Biomacromolecules
• 出版年：2017
• 出版时间：February 13, 2017
• 年：2017
• 卷：18
• 期：2
• 页码：413-421
• 全文大小：644K
• ISSN：1526-4602

文摘

Polymer-based biomaterials particularly polycaprolactone (PCL) are one of the most promising substrates for tissue engineering. The surface chemistry of these materials plays a major role since it governs protein adsorption, cell adhesion, viability, degradation, and biocompatibility in the first place. This study correlates the interaction of the most abundant serum proteins (albumin, immunoglobulins, fibrinogen) with the surface properties of PCL and its influence on the morphology and metabolic activity of primary human arterial endothelial cells that are seeded on the materials. Prior to that, thin films of PCL are manufactured by spin-coating and characterized in detail. A quartz crystal microbalance with dissipation (QCM-D), a multiparameter surface plasmon resonance spectroscopy instrument (MP-SPR), wettability data, and atomic force microscopy are combined to elucidate the pH-dependent protein adsorption on the PCL substrates. Primary endothelial cells are cultured on the protein modified polymer, and conclusions are drawn on the significant impact of type and form of proteins coatings on cell morphology and metabolic activity.