Development of a Solid-Phase Extraction Coupling Chemiluminescent Enzyme Immunoassay for Determination of Organophosphorus Pesticides in Environmental Water Samples
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- 作者：Zhen-Lin Xu ; Wen-Jia Sun ; Jin-Yi Yang ; Yue-Ming Jiang ; Katrina Campbell ; Yu-Dong Shen ; Hong-Tao Lei ; Dao-Ping Zeng ; Hong Wang ; Yuan-Ming Sun
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：March 7, 2012
- 年：2012
- 卷：60
- 期：9
- 页码：2069-2075
- 全文大小：230K
- 年卷期：v.60,no.9(March 7, 2012)
- ISSN：1520-5118
文摘
Solid-phase extraction (SPE) and direct competitive chemiluminescence enzyme immunoassay (dcCL-EIA) were combined for the detection of organophosphorus pesticides (OPs) in environmental water samples. dcCL-EIA based on horseradish peroxidase labeled with a broad-specificity monoclonal antibody against OPs was developed, and the effects of several physicochemical parameters on dcCL-EIA performance were studied. SPE was used for the pretreatment of water samples to remove interfering substances and to concentrate the OP analytes. The coupling of SPE and dcCL-EIA can detect seven OPs (parathion, coumaphos, phoxim, quinalphos, triazophos, dichlofenthion, and azinphos-ethyl) with the limit of quantitation below 0.1 ng/mL. The recoveries of OPs from spiked water samples ranged from 62.5% to 131.7% by SPE鈥揹cCL-EIA and 69.5% to 112.3% by SPE鈥揌PLC鈥揗S/MS. The screening of OP residues in real-world environmental water samples by the developed SPE鈥揹cCL-EIA and their confirmatory analysis using SPE鈥揌PLC鈥揗S/MS demonstrated that the assay is ideally suited as a monitoring method for OP residues prior to chromatographic analysis.