文摘
Microbial population and bioactive amine profile and levels of two lupin species (Lupinus luteus L.cv. 4492 and Lupinus angustifolius L. var. zapaton) and fenugreek (Trigonella foenum-graecum L.)seeds as affected by germination were investigated. Microbial population increased considerablymainly in the first stage of germination (2 days), then small changes in bacterial numbers wereobserved up to 5 days to levels between 7.8 and 8.9 log colony-forming units/g. Microorganismsbelonging to the Enterobacteriaceae family were dominant for the legumes tested. Ungerminatedlegume seeds contained putrescine, cadaverine, histamine, tyramine, spermidine, and spermine.Bioactive amine levels found in fenugreek seeds were between 3- and 4-fold higher than those foundin lupin seeds. The highest total amine levels were found in fenugreek seeds [162 mg/kg of dryweight (dw)], followed by L. angustifolius var. zapaton seeds (84 mg/kg of dw) and, finally, L. luteuscv. 4492 (46 mg/kg of dw) seeds. The concentration of individual amines showed a gradual risingtrend during the germination period in all tested sprouts, reaching levels >3 times higher than thosefound in ungerminated seeds. After 5 days of germination, the fenugreek sprouts contained the highestamount of total bioactive amines. Tyramine was the predominant amine in both lupin varieties, whereascadaverine was the main bioactive amine detected in fenugreek. The results of this work thus indicatedthat microbial population and biogenic amine levels in the studied lupin and fenugreek sprouts arenot a risk for healthy consumers or for individuals with restricted activity of detoxification enzymes.