Induction of de Novo Volatile Terpene Biosynthesis via Cytosolic and Plastidial Pathways by Methyl Jasmonate in Foliage of Vitis vinifera L.
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- 作者：Daniela Hampel ; Armin Mosandl ; and Matthias Wü ; st
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2005
- 出版时间：April 6, 2005
- 年：2005
- 卷：53
- 期：7
- 页码：2652 - 2657
- 全文大小：86K
- 年卷期：v.53,no.7(April 6, 2005)
- ISSN：1520-5118
文摘
The terpene biosynthesis in leaves of Vitis vinifera L. cv. Morio Muskat was studied using methyljasmonate to induce defensive responses in vivo. The experiments demonstrated the strong activationof the de novo biosynthesis of terpenoids via the octadecanoid-signaling cascade and release of thecompounds to the gas phase. Feeding experiments with [5,5-2H2]-1-deoxy-D-xylulose and [5,5-2H2]mevalonic acid lactone allowed the investigation of the dynamic allocation of resources via themevalonic acid and 1-deoxy-D-xylulose/2-C-methyl-D-erythritol 4-phosphate (DOXP/MEP) pathwayunder induced conditions and after treatment with the specific inhibitors mevastatin and fosmidomycin.The experiments reveal that monoterpenes are almost exclusively synthesized via the DOXP/MEPpathway, whereas sesquiterpenes are generated via both pathways at approximately equal rates.The biosynthesis of the homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene was not affected bymevastatine or fosmidomycin.Keywords: Vitaceae; 1-deoxy-D-xylulose; mevalonic acid; inhibition studies; octadecanoid-signalingcascade; enantioselective multidimensional gas chromatography-mass spectrometry (enantio-MDGC-MS); dynamic headspace extraction