Functional Characterization of the Cyclomarin/Cyclomarazine Prenyltransferase CymD Directs the Biosynthesis of Unnatural Cyclic Peptides
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- 作者：Andrew W. Schultz ; Chad A. Lewis ; Michael R. Luzung ; Phil S. Baran ; Bradley S. Moore
- 刊名：Journal of Natural Products
- 出版年：2010
- 出版时间：March 26, 2010
- 年：2010
- 卷：73
- 期：3
- 页码：373-377
- 全文大小：178K
- 年卷期：v.73,no.3(March 26, 2010)
- ISSN：1520-6025
文摘
In vitro and in vivo characterization of the cyclomarin/cyclomarazine prenyltransferase CymD revealed its ability to prenylate tryptophan prior to incorporation into both cyclic peptides by the nonribosomal peptide synthetase CymA. This knowledge was utilized to bioengineer novel derivatives of these marine bacterial natural products by providing synthetic N-alkyl tryptophans to a prenyltransferase-deficient mutant of Salinispora arenicola CNS-205.