Transient Tertiary Structure Formation within the Ribosome Exit Port

详细信息    查看全文

• 作者：Edward P. O’ ; Brien ; Shang-Te Danny Hsu ; John Christodoulou ; Michele Vendruscolo ; Christopher M. Dobson
• 刊名：Journal of the American Chemical Society
• 出版年：2010
• 出版时间：December 1, 2010
• 年：2010
• 卷：132
• 期：47
• 页码：16928-16937
• 全文大小：587K
• 年卷期：v.132,no.47(December 1, 2010)
• ISSN：1520-5126

文摘

The exit tunnel of the ribosome is commonly considered to be sufficiently narrow that co-translational folding can begin only when specific segments of nascent chains are fully extruded from the tunnel. Here we show, on the basis of molecular simulations and comparison with experiment, that the long-range contacts essential for initiating protein folding can form within a nascent chain when it reaches the last 20 Å of the exit tunnel. We further show that, in this “exit port”, a significant proportion of native and non-native tertiary structure can form without steric overlap with the ribosome itself, and provide a library of structural elements that our simulations predict can form in the exit tunnel and is amenable to experimental testing. Our results show that these elements of folded tertiary structure form only transiently and are at their midpoints of stability at the boundary region between the inside and the outside of the tunnel. These findings provide a framework for interpreting a range of recent experimental studies of ribosome nascent chain complexes and for understanding key aspects of the nature of co-translational folding.