Highly-Branched Poly(N-isopropylacrylamide) as a Component in Poly(dopamine) Films

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• 作者：Yan Zhang ; Boon M. Teo ; Almar Postma ; Francesca Ercole ; Ryosuke Ogaki ; Meifang Zhu ; Brigitte St盲dler
• 刊名：Journal of Physical Chemistry B
• 出版年：2013
• 出版时间：September 12, 2013
• 年：2013
• 卷：117
• 期：36
• 页码：10504-10512
• 全文大小：447K
• 年卷期：v.117,no.36(September 12, 2013)
• ISSN：1520-5207

文摘

Mixed one-step poly(dopamine) (PDA)/highly branched poly(N-isopropylacrylamide) (pNiPAAm) coatings have been assembled and characterized by X-ray photoelectron spectroscopy (XPS), UV鈥搗is spectroscopy, atomic force microscopy, and quartz crystal microbalance with dissipation monitoring (QCM-D) depending on the deposition temperature below and above the lower critical solution temperature (LCST) of the pNiPAAm. Mixed films were confirmed. The protein adsorption at 24 掳C was found to be reduced with increasing amount of pNiPAAm in the mixed coatings, while there was no difference observed for proteins deposition at 39 掳C. Further, the ability of these mixed coatings in comparison to the pure PDA and pNiPAAm films to serve as capping layer for surface-immobilized zwitterionic or positively charged liposomes has been assessed by QCM-D. The adhesion of hepatocytes, macrophages, and myoblast to these liposomes-containing hybrid coatings and the uptake of fluorescent lipids from the surface by the adhering cells depending on the capping layers were compared. The latter aspect was found to be dependent on the used capping layer and the type of liposome as carrier for the fluorescent lipid, with the highest uptake found for positive liposomes and pure pNiPAAm as capping layer. Taken together, the assembled hybrid coatings have the potential to be used as functional coatings toward surface-mediated drug delivery.