Interaction of Bovine Serum Albumin with Cationic Single Chain+Nonionic and Cationic Gemini+Nonionic Binary Surfactant Mixtures

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• 作者：Mohammad Amin Mir ; Nuzhat Gull ; Javeed Masood Khan ; Rizwan Hasan Khan ; Aijaz Ahmad Dar ; Ghulam Mohammad Rather
• 刊名：Journal of Physical Chemistry B
• 出版年：2010
• 出版时间：March 11, 2010
• 年：2010
• 卷：114
• 期：9
• 页码：3197-3204
• 全文大小：291K
• 年卷期：v.114,no.9(March 11, 2010)
• ISSN：1520-5207

文摘

The interaction of bovine serum albumin (BSA) with cetyltrimethylammonium bromide (CTAB), C₁₆C₄C₁₆Br₂, Brij58, and their binary mixtures has been studied using tensiometry, spectrofluorometry, and circular dichroism at physiological pH and 25 °C. The tensiometric profiles of CTAB and C₁₆C₄C₁₆Br₂ in the presence of BSA exhibit a single break at a lower surfactant concentration termed as C₁ (concentration corresponding to saturation of the interface) compared to their critical micelle concentration (CMC) in the buffered solution. However, for Brij58, CTAB+Brij58, and C₁₆C₄C₁₆Br₂+Brij58, two breaks were observed, first at the critical aggregation concentraion (CAC), corresponding to onset of interaction with BSA and the second at C₁ corresponding to saturation of the interface. The interaction of CTAB+Brij58 and C₁₆C₄C₁₆Br₂+Brij58 mixtures with the BSA solution is discussed in terms of competition between surfactant−surfactant and surfactant−BSA interactions. CTAB+Brij58 and C₁₆C₄C₁₆Br₂+Brij58 mixtures show nonideality with respect to mixed micelle formation, which is reflected in their interaction with the BSA. The interaction of CTAB+Brij58 with BSA decreases with increase in the mole fraction of CTAB in the mixture, whereas in C₁₆C₄C₁₆Br₂+Brij58 the reverse is the case. The results of the present study may prove fruitful in optimizing the properties of surfactant−protein mixtures relevant for many formulations.