Interaction and Comparison of a Class I Hydrophobin from Schizophyllum commune and Class II Hydrophobins from Trichoderma reesei

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• 作者：Sanna Askolin ; Markus Linder ; Karin Scholtmeijer ; Maija Tenkanen ; Merja Penttil&auml ; Marcel L. de Vocht ; and Han A. B. Wö ; sten
• 刊名：Biomacromolecules
• 出版年：2006
• 出版时间：April 2006
• 年：2006
• 卷：7
• 期：4
• 页码：1295 - 1301
• 全文大小：449K
• 年卷期：v.7,no.4(April 2006)
• ISSN：1526-4602

文摘

Hydrophobins fulfill a wide spectrum of functions in fungal growth and development. These proteins self-assembleat hydrophilic-hydrophobic interfaces into amphipathic membranes. Hydrophobins are divided into two classesbased on their hydropathy patterns and solubility. We show here that the properties of the class II hydrophobinsHFBI and HFBII of Trichoderma reesei differ from those of the class I hydrophobin SC3 of Schizophyllumcommune. In contrast to SC3, self-assembly of HFBI and HFBII at the water-air interface was neither accompaniedby a change in secondary structure nor by a change in ultrastructure. Moreover, maximal lowering of the watersurface tension was obtained instantly or took several minutes in the case of HFBII and HFBI, respectively. Incontrast, it took several hours in the case of SC3. Oil emulsions prepared with HFBI and SC3 were more stablethan those of HFBII, and HFBI and SC3 also interacted more strongly with the hydrophobic Teflon surfacemaking it wettable. Yet, the HFBI coating did not resist treatment with hot detergent, while that of SC3 remainedunaffected. Interaction of all the hydrophobins with Teflon was accompanied with a change in the circular dichroismspectra, indicating the formation of an -helical structure. HFBI and HFBII did not affect self-assembly of theclass I hydrophobin SC3 of S. commune and vice versa. However, precipitation of SC3 was reduced by the classII hydrophobins, indicating interaction between the assemblies of both classes of hydrophobins.