Control of Protein Orientation on Gold Nanoparticles

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• 作者：Wayne Lin ; Thomas Insley ; Marcus D. Tuttle ; Lingyang Zhu ; Deborah A. Berthold ; Petr Kr谩l ; Chad M. Rienstra ; Catherine J. Murphy
• 刊名：Journal of Physical Chemistry C
• 出版年：2015
• 出版时间：September 10, 2015
• 年：2015
• 卷：119
• 期：36
• 页码：21035-21043
• 全文大小：520K
• ISSN：1932-7455

文摘

Gold nanoparticles (Au NPs) have attracted much attention due to their potential applications in nanomedicine. While numerous studies have quantified biomolecular adsorption to Au NPs in terms of equilibrium binding constants, far less is known about biomolecular orientation on nanoparticle surfaces. In this study, the binding of the protein 伪-synuclein to citrate and (16-mercaptohexadecyl)trimethylammonium bromide (MTAB)-coated 12 nm Au NPs is examined by heteronuclear single quantum coherence NMR spectroscopy to provide site-specific measurements of protein鈥搉anoparticle binding. Molecular dynamics simulations support the orientation assignments, which show N-terminus binding to the Au NP for citrate-capped NPs and C-terminus binding for the MTAB-capped NPs.