Antibody-Free Colorimetric Detection of Total Aflatoxins in Rice Based on a Simple Two-Step Chromogenic Reaction

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• 作者：Bibai Du ; Xiaoou Su ; Kunhao Yang ; Long Pan ; Qingju Liu ; Lingling Gong ; Peilong Wang ; Jingkui Yang ; Yujian He
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：April 5, 2016
• 年：2016
• 卷：88
• 期：7
• 页码：3775-3780
• 全文大小：373K
• ISSN：1520-6882

文摘

The prevalently used immunoassays for fast screening of aftatoxins (AFs) usually cannot meet the requirement for simultaneous determination of total AFs (aflatoxin B₁ + aflatoxin B₂ + aflatoxin G₁ + aflatoxin G₂) due to the deficiency of highly group-specific antibodies. This paper describes a two-step chromogenic reaction based method to quantitatively detect total AFs in rice using colorimetric measurement without antibody. In the method, colorless AFs transform into green-colored indophenol products through the reaction with sodium hydroxide and 2,6-dibromoquinone-4-chloroimide (DBQC) successively, allowing selectively determining total AFs up to 3.9 μg/kg over other competitive mycotoxins under optimal conditions by a UV–vis spectrophotometer. In addition, the colorimetric measurement results of the rice samples agree well with that of a standard HPLC method, demonstrating the good reliability and applicability of the method. Uniquely, the method has potential for on-site detection of total AFs in rice when using a nylon membrane-based device.