Disease Detection by Ultrasensitive Quantification of Microdosed Synthetic Urinary Biomarkers

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• 作者：Andrew D. Warren ; Shonda T. Gaylord ; Kevin C. Ngan ; Milena Dumont Milutinovic ; Gabriel A. Kwong ; Sangeeta N. Bhatia ; David R. Walt
• 刊名：Journal of the American Chemical Society
• 出版年：2014
• 出版时间：October 1, 2014
• 年：2014
• 卷：136
• 期：39
• 页码：13709-13714
• 全文大小：381K
• ISSN：1520-5126

文摘

The delivery of exogenous agents can enable noninvasive disease monitoring, but existing low-dose approaches require complex infrastructure. In this paper, we describe a microdose-scale injectable formulation of nanoparticles that interrogate the activity of thrombin, a key regulator of clotting, and produce urinary reporters of disease state. We establish a customized single molecule detection assay that enables urinary discrimination of thromboembolic disease in mice using doses of the nanoparticulate diagnostic agents that fall under regulatory guidelines for 鈥渕icrodosing.鈥?/p>