Exploring the Catalytic Promiscuity of a New Glycosyltransferase from Carthamus tinctorius

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• 作者：Kebo Xie ; Ridao Chen ; Jianhua Li ; Ruishan Wang ; Dawei Chen ; Xiaoxiang Dou ; Jungui Dai
• 刊名：Organic Letters
• 出版年：2014
• 出版时间：September 19, 2014
• 年：2014
• 卷：16
• 期：18
• 页码：4874-4877
• 全文大小：332K
• ISSN：1523-7052

文摘

The catalytic promiscuity of a new glycosyltransferase (UGT73AE1) from Carthamus tinctorius was explored. UGT73AE1 showed the capability to glucosylate a total of 19 structurally diverse types of acceptors and to generate O-, S-, and N-glycosides, making it the first reported trifunctional plant glycosyltransferase. The catalytic reversibility and regioselectivity were observed and modeled in a one-pot reaction transferring a glucose moiety from icariin to emodin. These findings demonstrate the potential versatility of UGT73AE1 in the glycosylation of bioactive natural products.