Peroxynitrite Scavenging Activity of Sinapic Acid (3,5-Dimethoxy-4-hydroxycinnamic Acid) Isolated from Brassica juncea

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• 作者：Yani Zou ; Ae Ra Kim ; Jung Eun Kim ; Jae Sue Choi ; and Hae Young Chung
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2002
• 出版时间：October 9, 2002
• 年：2002
• 卷：50
• 期：21
• 页码：5884 - 5890
• 全文大小：160K
• 年卷期：v.50,no.21(October 9, 2002)
• ISSN：1520-5118

文摘

Peroxynitrite (ONOO^-), formed from a reaction of superoxide and nitric oxide, is one of the mostpotent cytotoxic species that are known to oxidize cellular constituents including essential proteins,lipids, and DNA. In this study, the ability of sinapic acid (3,5-dimethoxy-4-hydroxycinnamic acid),isolated from Brassica juncea, to scavenge ONOO^- was investigated. The data obtained show thatsinapic acid can efficiently scavenge native ONOO^- as well as ONOO^- derived from the peroxynitritedonor 3-morpholinosydnonimine hydrochloride (SIN-1). Spectrophotometric analyses revealed thatsinapic acid suppressed the formation of ONOO^--mediated tyrosine nitration through an electrondonation mechanism. In further studies, sinapic acid also showed a significant ability of inhibitingnitration of bovine serum albumin and low-density lipoprotein (LDL) in a dose-dependent manner.Sinapic acid decreased the LDL peroxidation induced by SIN-1-derived ONOO^-. The present studysuggests that sinapic acid has an efficient ONOO^- scavenging ability, which may well be a potentONOO^- oxidant scavenger for the protection of the cellular defense activity against the ONOO^--involved diseases.Keywords: Brassica juncea; sinapic acid; 3,5-dimethoxy-4-hydroxycinnamic acid; nitrotyrosine; ONOO^-scavenging activity; lipid peroxidation