Structure of a Precursor to the Blue Components Produced in the Blue Discoloration in Japanese Radish (Raphanus sativus) Roots
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- 作者：Katsunori Teranishi ; Nagata Masayasu
- 刊名：Journal of Natural Products
- 出版年：2016
- 出版时间：May 27, 2016
- 年：2016
- 卷：79
- 期：5
- 页码：1381-1387
- 全文大小：389K
- 年卷期：0
- ISSN：1520-6025
文摘
The internal blue discoloration in Japanese radish (Raphanus sativus L.) roots has been reported to be a physiological phenomenon after harvest and poses a significant problem for farmers. To avoid this discoloration, the fundamental development of new radish cultivars that do not undergo discoloration and/or improved cultivation methods is required. Elucidating the chemical mechanism leading to this discoloration could help overcome these difficulties. To determine the mechanism underlying this discoloration, this study was designed to probe the structure of a precursor to the blue components generated during the discoloration process. Soaking fresh roots in aqueous H2O2 resulted in rapid blue discoloration, similar to the natural discoloration. Using a H2O2-based blue discoloration assay, the precursor was extracted and isolated from the fresh roots and identified as the glucosinolate, 4-hydroxyglucobrassicin, via spectroscopy and chemical synthesis.