Spraying Quantum Dot Conjugates in the Colon of Live Animals Enabled Rapid and Multiplex Cancer Diagnosis Using Endoscopy
详细信息 查看全文
- 作者：Youngrong Park ; Yeon-Mi Ryu ; Yebin Jung ; Taejun Wang ; Yeonggyeong Baek ; Yeoreum Yoon ; Sang Mun Bae ; Joonhyuck Park ; Sekyu Hwang ; Jaeil Kim ; Eun-Ju Do ; Sang-Yeob Kim ; Euiheon Chung ; Ki Hean Kim ; Sungjee Kim ; Seung-Jae Myung
- 刊名：ACS Nano
- 出版年：2014
- 出版时间：September 23, 2014
- 年：2014
- 卷：8
- 期：9
- 页码：8896-8910
- 全文大小：1023K
- ISSN：1936-086X
文摘
The detection of colon cancer using endoscopy is widely used, but the interpretation of the diagnosis is based on the clinician鈥檚 naked eye. This is subjective and can lead to false detection. Here we developed a rapid and accurate molecular fluorescence imaging technique using antibody-coated quantum dots (Ab鈥換Ds) sprayed and washed simultaneously on colon tumor tissues inside live animals, subsequently excited and imaged by endoscopy. QDs were conjugated to matrix metalloproteinases (MMP) 9, MMP 14, or carcinoembryonic antigen (CEA) Abs with zwitterionic surface coating to reduce nonspecific bindings. The Ab鈥換D probes can diagnose tumors on sectioned mouse tissues, fresh mouse colons stained ex vivo and also in vivo as well as fresh human colon adenoma tissues in 30 min and can be imaged with a depth of 100 渭m. The probes successfully detected not only cancers that are readily discernible by bare eyes but also hyperplasia and adenoma regions. Sum and cross signal operations provided postprocessed images that can show complementary information or regions of high priority. This multiplexed quantum dot, spray-and-wash, and endoscopy approach provides a significant advantage for detecting small or flat tumors that may be missed by conventional endoscopic examinations and bestows a strategy for the improvement of cancer diagnosis.