Long-Term Maintenance of a Transgenic Catharanthus roseus Hairy Root Line

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• 作者：Christie A. M. Peebles ; Susan I. Gibson ; Jacqueline V. Shanks ; Ka-Yiu San
• 刊名：Biotechnology Progress
• 出版年：2007
• 出版时间：December 2007
• 年：2007
• 卷：23
• 期：6
• 页码：1517 - 1518
• 全文大小：54K
• 年卷期：v.23,no.6(December 2007)
• ISSN：1520-6033

文摘

Stably transformed transgenic hairy root cultures have the potential to be a valuable productionplatform for a variety of secondary metabolites. This study reports that a transgenic hairy rootculture of Catharanthus roseus has been stably maintained for over 4.5 years. This culture carriesa transgene that expresses the green fluorescent protein under the control of the glucocorticoid-inducible promoter. Genomic PCR confirmed the presence of the GFP insert within the hairyroots, and induction with dexamethasone caused a significant (p < 0.02) increase in GFP levels.