Activity, Expression, and Substrate Preference of the Δ6-Desaturase in Slow- or Fast-Growing Rabbit Genotypes
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- 作者：Cesare Castellini ; Alessandro Dal Bosco ; Simona Mattioli ; Magdalena Davidescu ; Lanfranco Corazzi ; Lara Macchioni ; Simona Rimoldi ; Genciana Terova
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2016
- 出版时间：February 3, 2016
- 年：2016
- 卷：64
- 期：4
- 页码：792-800
- 全文大小：588K
- ISSN：1520-5118
文摘
In the present paper liver fatty acid Δ6 desaturation (fads2) activity was analyzed in two rabbit strains with slow- (S, 27.5 g/day) or fast-growing (F, 48.5 g/day) rate. The fatty acid profile of the liver showed a different PUFA profile in the two strains with a lower n-6/n-3 ratio in the S rabbits. The expression of fads2 was 2-fold higher in S than in F rabbits, whereas enzyme activity was higher in F and more oriented toward the desaturation of linoleic acid (90%). In contrast, S showed a higher preference for linolenic acid (38.9 vs 10%). This study identified a single difference in the fads2 amino acid sequence between these two strains. Such a difference consists in the substitution of Gly104 to Ser104 in the sequence of F fads2. These results indicate for the first time that genetic selection for performance may affect the preference for PUFA toward desaturation of linoleic/linolenic acid.