Effect of Steam Treatment of Alperujo on the Composition, Enzymatic Saccharification, and in Vitro Digestibility of Alperujo
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- 作者：Guillermo Rodrí ; guez ; Rocí ; o Rodrí ; guez ; Ana Jimé ; nez ; Rafael Guillé ; n ; Juan Ferná ; ndez-Bolañ ; os
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：January 10, 2007
- 年：2007
- 卷：55
- 期：1
- 页码：136 - 142
- 全文大小：73K
- 年卷期：v.55,no.1(January 10, 2007)
- ISSN：1520-5118
文摘
The solid waste from two-phase olive oil extraction or "alperujo" was submitted to steam treatmentat high pressure or temperature, 200 
C for 5 min, in the presence and absence of mild acid catalyst.This treatment made easier the separation of the solid and liquid fractions. Besides the recovery ofcertain valuable components from the liquid fraction (the antioxidant hydroxytyrosol, low molecularweight oligosaccharides, glucose, mannitol, etc.), the major components of the solid residue couldbe also exploited. In this study, changes in composition of alperujo due to steam treatment weredetermined. The process reduced appreciably the hemicellulose concentrations (75-88%), removeda substantial portion of Klason lignin and protein (50%), and led to an extensive solubilization ofalperujo (55-67%). Cellulose was very resistant to autohydrolysis and acid-catalyzed hydrolysis, sothe solid residue was enriched in fat (13-18 g/100 g of dry steam-treated alperujo) and cellulose(15-25 g/100 g of dry and defatted steam-treated alperujo). The steam-treated material can beefficiently saccharified with commercial cellulase. The best hydrolysis yields were attained, up to80%, when the treated material was post-treated with NaOH. The possibility of using this steam-treated alperujo in animal feeding was evaluated by an in vitro digestibility test, using the pepsin-cellulase method. The treatment affected positively the nutritional characteristics of alperujo with anincrease in its in vitro (dry and organic matter) digestibility (8-10% higher than untreated material).In vitro digestibility and cellulose accessibility to enzymatic hydrolysis were improved by the alkalipost-treatment.
C for 5 min, in the presence and absence of mild acid catalyst.This treatment made easier the separation of the solid and liquid fractions. Besides the recovery ofcertain valuable components from the liquid fraction (the antioxidant hydroxytyrosol, low molecularweight oligosaccharides, glucose, mannitol, etc.), the major components of the solid residue couldbe also exploited. In this study, changes in composition of alperujo due to steam treatment weredetermined. The process reduced appreciably the hemicellulose concentrations (75-88%), removeda substantial portion of Klason lignin and protein (50%), and led to an extensive solubilization ofalperujo (55-67%). Cellulose was very resistant to autohydrolysis and acid-catalyzed hydrolysis, sothe solid residue was enriched in fat (13-18 g/100 g of dry steam-treated alperujo) and cellulose(15-25 g/100 g of dry and defatted steam-treated alperujo). The steam-treated material can beefficiently saccharified with commercial cellulase. The best hydrolysis yields were attained, up to80%, when the treated material was post-treated with NaOH. The possibility of using this steam-treated alperujo in animal feeding was evaluated by an in vitro digestibility test, using the pepsin-cellulase method. The treatment affected positively the nutritional characteristics of alperujo with anincrease in its in vitro (dry and organic matter) digestibility (8-10% higher than untreated material).In vitro digestibility and cellulose accessibility to enzymatic hydrolysis were improved by the alkalipost-treatment.