Grape-Seed Procyanidins Act as Antiinflammatory Agents in Endotoxin-Stimulated RAW 264.7 Macrophages by Inhibiting NFkB Signaling Pathway
详细信息 查看全文
- 作者：Ximena Terra ; Josep Valls ; Xavier Vitrac ; Jean-Michel Mé ; rrillon ; Lluí ; s Arola ; Anna Ardevol ; Cinta Bladé ; Juan Ferná ; ndez-Larrea ; Gerard Pujadas ; Josepa Salvadó ; Mayte
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：May 30, 2007
- 年：2007
- 卷：55
- 期：11
- 页码：4357 - 4365
- 全文大小：386K
- 年卷期：v.55,no.11(May 30, 2007)
- ISSN：1520-5118
文摘
Procyanindin extract (PE) is a mixture of polyphenols, mainly procyanidins, obtained from grape seedwith putative antiinflammatory activity. We evaluated the PE effect on RAW 264.7 macrophagesstimulated with lipopolysaccharide plus interferon-
that show a rapid enhanced production ofprostaglandin E2 (PGE2) and nitric oxide (NO). Our results demonstrated that PE significantly inhibitedthe overproduction of NO, dose and time dependently. PE caused a marked inhibition of PGE2synthesis when administered during activation. Moreover, PE pretreatment diminished iNOS mRNAand protein amount dose dependently (10-65 
g/mL). PE (65 g/mL) pretreatment inhibited NF
B(p65) translocation to nucleus by nearly 40%. Trimeric and longer oligomeric-rich procyanidin fractionsfrom PE (5-30 g/mL) inhibited iNOS expression but not the monomeric forms catechin andepicatechin. Thus, we show that the degree of polymerization is important in determining procyanidineffects. PE was considerably a more effective inhibitor of NO biosynthesis (IC50 = 50 g/mL) incomparison to other antiinflammatories, such as aspirin (3 mM), indomethacin (20 M), anddexamethasone (9 nM). In conclusion, PE modulates inflammatory response in activated macrophagesby the inhibition of NO and PGE2 production, suppression of iNOS expression, and NFkB translocation.These results demonstrate an immunomodulatory role of grape seed procyanidins and thus a potentialhealth-benefit in inflammatory conditions that exert an overproduction of NO and PGE2.
that show a rapid enhanced production ofprostaglandin E2 (PGE2) and nitric oxide (NO). Our results demonstrated that PE significantly inhibitedthe overproduction of NO, dose and time dependently. PE caused a marked inhibition of PGE2synthesis when administered during activation. Moreover, PE pretreatment diminished iNOS mRNAand protein amount dose dependently (10-65 g/mL). PE (65 g/mL) pretreatment inhibited NFB(p65) translocation to nucleus by nearly 40%. Trimeric and longer oligomeric-rich procyanidin fractionsfrom PE (5-30 g/mL) inhibited iNOS expression but not the monomeric forms catechin andepicatechin. Thus, we show that the degree of polymerization is important in determining procyanidineffects. PE was considerably a more effective inhibitor of NO biosynthesis (IC50 = 50 g/mL) incomparison to other antiinflammatories, such as aspirin (3 mM), indomethacin (20 M), anddexamethasone (9 nM). In conclusion, PE modulates inflammatory response in activated macrophagesby the inhibition of NO and PGE2 production, suppression of iNOS expression, and NFkB translocation.These results demonstrate an immunomodulatory role of grape seed procyanidins and thus a potentialhealth-benefit in inflammatory conditions that exert an overproduction of NO and PGE2.