Apoptotic Effect of Quercetin on HT-29 Colon Cancer Cells via the AMPK Signaling Pathway
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- 作者：Hyeong-Jin Kim ; Sang-Ki Kim ; Byeong-Soo Kim ; Seung-Ho Lee ; Young-Seok Park ; Byung-Kwon Park ; So-Jung Kim ; Jin Kim ; Changsun Choi ; Jong-Suk Kim ; Sung-Dae Cho ; Ji-Won Jung ; Kyong-Hwan Roh ; Kyung-Sun Kan
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2010
- 出版时间：August 11, 2010
- 年：2010
- 卷：58
- 期：15
- 页码：8643-8650
- 全文大小：997K
- 年卷期：v.58,no.15(August 11, 2010)
- ISSN：1520-5118
文摘
Activation of AMP-activated protein kinase (AMPK), a physiological cellular energy sensor, strongly suppresses cell proliferation in both nonmalignant and tumor cells. This study demonstrates the mechanism of quercetin-induced apoptosis in HT-29 colon cancer cells. Treatment of cells with quercetin significantly decreased cell viability in a dose-dependent manner. Notably, quercetin increased cell cycle arrest in the G1 phase and up-regulated apoptosis-related proteins, such as AMPK, p53, and p21, within 48 h. Furthermore, in vivo experiments showed that quercetin treatment resulted in a significant reduction in tumor volume over 6 weeks, and apoptosis-related protein induction by quercetin was significantly higher in the 100 mg/kg treated group compared to the control group. All of these results indicate that quercetin induces apoptosis via AMPK activation and p53-dependent apoptotic cell death in HT-29 colon cancer cells and that it may be a potential chemopreventive or therapeutic agent against HT-29 colon cancer.