Enzymatic Synthesis of Fluorinated Mechanistic Probes for Sialidases and Sialyltransferases

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• 作者：Harshal A. Chokhawala ; Hongzhi Cao ; Hai Yu ; Xi Chen
• 刊名：Journal of the American Chemical Society
• 出版年：2007
• 出版时间：September 5, 2007
• 年：2007
• 卷：129
• 期：35
• 页码：10630 - 10631
• 全文大小：85K
• 年卷期：v.129,no.35(September 5, 2007)
• ISSN：1520-5126

文摘

A general, convenient, and efficient enzymatic approach for producing fluorinated mechanistic probes for sialidases and sialyltransferases is described. Other than the reported sialyltransferase inhibitor CMP-3F(axial)Neu5Ac, CMP-3F(equatorial)Sia derivatives (in which Sia is Neu5Ac or Neu5Gc) have been synthesized along with CMP-3F(axial)Neu5Gc and CMP-3F(axial)KDN as novel inhibitors and important mechanistic probes for sialyltransferases. 3-Fluoro-sialosides have been enzymatically synthesized and could be used as potential inhibitors and mechanistic probes for sialidases and as probes for sialic acid-binding proteins.