Receptor Arrays for the Selective and Efficient Capturing of Viral Particles
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- 作者：Philipp D. Pollheimer† ; ‡ ; ; Markus Kastner† ; ; Andreas Ebner† ; ; Dieter Blaas§ ; ; Peter Hinterdorfer† ; ; Herma
- 刊名：Bioconjugate Chemistry
- 出版年：2009
- 出版时间：March 18, 2009
- 年：2009
- 卷：20
- 期：3
- 页码：466-475
- 全文大小：398K
- 年卷期：v.20,no.3(March 18, 2009)
- ISSN：1520-4812
文摘
We describe microarrays of receptors on gold/glass substrates for the selective capturing of viral particles at high density. Microscale gold squares were surface-modified with alkanethiol derivatives which enabled the immobilization of the His6-tagged virus-binding domain from the very-low density lipoprotein (VLDL) receptor. The free glass areas surrounding the gold squares were passivated with a dense film of poly(ethylene glycol) (PEG). As assessed by atomic force microscopy, human rhinovirus particles were captured onto the VLDL-receptor patches with a high surface coverage but were effectively repelled by the PEG layer, resulting in a 330000-fold higher density of the particles on the gold as compared to the glass surfaces. The metal chelate-based coupling strategy was found to be superior to two alternative routes, which used the covalent coupling of viral particles or viral receptors to the substrate surface. The high-density receptor arrays were employed for sensing and characterizing viral particles with so far unprecedented selectivity.