Photoinduced Cross-Linking of Short Furan-Modified DNA on Surfaces

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• 作者：Cinthya Véliz Montes ; Henry Memczak ; Ellen Gyssels ; Tomás Torres ; Annemieke MadderRudolf J. Schneider
• 刊名：Langmuir
• 出版年：2017
• 出版时间：February 7, 2017
• 年：2017
• 卷：33
• 期：5
• 页码：1197-1201
• 全文大小：321K
• ISSN：1520-5827

文摘

We report for the first time the formation of site-specific interstrand cross-linked (ICL) surface-immobilized furan-modified DNA duplexes via singlet oxygen. <sup>1sup>O<sub>2sub>, necessary for effecting furan-mediated ICL formation, was produced in situ using methylene blue or a zinc phthalocyanine derivative (TT1) as a photosensitizer. Via surface plasmon resonance spectroscopy, we show that surface ICL was achieved, and a robust link formed that enhances the stability of the 12-mer duplex even after surface regeneration. The described method represents a novel platform technology based on surfaces with addressable and stable DNA duplexes requiring only short oligonucleotides.