Highly Water-Soluble BODIPY-Based Fluorescent Probe for Sensitive and Selective Detection of Nitric Oxide in Living Cells
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- 作者：Giri K. Vegesna ; Srinivas R. Sripathi ; Jingtuo Zhang ; Shilei Zhu ; Weilue He ; Fen-Tair Luo ; Wan Jin Jahng ; Megan Frost ; Haiying Liu
- 刊名：ACS Applied Materials & Interfaces
- 出版年：2013
- 出版时间：May 22, 2013
- 年：2013
- 卷：5
- 期：10
- 页码：4107-4112
- 全文大小：409K
- 年卷期：v.5,no.10(May 22, 2013)
- ISSN：1944-8252
文摘
A highly water-soluble BODIPY dye bearing electron-rich o-diaminophenyl groups at 2,6-positions was prepared as a highly sensitive and selective fluorescent probe for detection of nitric oxide (NO) in living cells. The fluorescent probe displays an extremely weak fluorescence with fluorescence quantum yield of 0.001 in 10 mM phosphate buffer (pH 7.0) in the absence of NO as two electron-rich o-diaminophenyl groups at 2,6-positions significantly quench the fluorescence of the BODIPY dye via photoinduced electron transfer mechanism. The presence of NO in cells enhances the dye fluorescence dramatically. The fluorescent probe demonstrates excellent water solubility, membrane permeability, and compatibility with living cells for sensitive detection of NO.