Biological Characterization of Folate-Decorated Biodegradable Polymer鈥揚latinum(II) Complex Micelles
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- 作者：Rui Wang ; Xiuli Hu ; Sai Wu ; Haihua Xiao ; Haidong Cai ; Zhigang Xie ; Yubin Huang ; Xiabin Jing
- 刊名：Molecular Pharmaceutics
- 出版年：2012
- 出版时间：November 5, 2012
- 年：2012
- 卷：9
- 期：11
- 页码：3200-3208
- 全文大小：438K
- 年卷期：v.9,no.11(November 5, 2012)
- ISSN：1543-8392
文摘
A biodegradable and amphiphilic copolymer, poly(ethylene glycol)-block-poly(l-lactide-co-2-methyl-2-carboxyl-propylene carbonate) (mPEG-b-P(LA-co-MCC)), which contains pendant carboxyl groups, was chosen as a drug carrier for the active anticancer part (diaminocyclohexane platinum, DACH-Pt) of oxaliplatin to form mPEG-b-P(LA-co-MCC/Pt) complex. A folic acid-conjugated copolymer, folic acid鈥損oly(ethylene glycol)-block-poly(l-lactide) (FA鈥揚EG-PLA), with similar chemical structure was chosen for targeting. Multifunctional micelles were successfully prepared by a coassembling method. In vitro evaluation was performed by using SKOV-3 and MCF-7 cancer cells. In vivo blood clearance of platinum was studied, and the results show that micelles exhibit longer blood circulation after iv injection. Pt biodistribution was studied by measuring its levels in plasma, organs, and tumors, especially in tumor cell DNA, by atomic absorption and inductively coupled plasma mass spectrometry. Antitumor activity was assessed in mice bearing H22 liver cancers, and the results showed that the micelles with FA moieties exhibited greater antitumor efficacy than those without FA or oxaliplatin. Therefore, these novel multifunctional platinum micelles have great potential in future clinical application.