Photoresponsive DNA-Cross-Linked Hydrogels for Controllable Release and Cancer Therapy

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• 作者：Huaizhi Kang ; Haipeng Liu ; Xiaoling Zhang ; Jilin Yan ; Zhi Zhu ; Lu Peng ; Huanghao Yang ; Youngmi Kim ; Weihong Tan
• 刊名：Langmuir
• 出版年：2011
• 出版时间：January 4, 2011
• 年：2011
• 卷：27
• 期：1
• 页码：399-408
• 全文大小：1100K
• 年卷期：v.27,no.1(January 4, 2011)
• ISSN：1520-5827

文摘

We have developed a photoresponsive DNA-cross-linked hydrogel that can be photoregulated by two wavelengths with a reversible sol鈭抔el conversion. This photoinduced conversion can be further untilized for precisely controllable encapsulation and release of multiple loads. Specifically, photosensitive azobenzene moieties are incorporated into DNA strands as cross-linkers, such that their hybridization to complementary DNAs (cDNAs) responds differently to different wavelengths of light. On the basis of the rheology variation of hydrogels, it is possible to utilize this material for storing and releasing molecules and nanoparticles. To prove the concept, three different materials鈥昮luorescein, horseradish peroxidase, and gold nanoparticles鈥晈ere encapsulated inside the gel at 450 nm and then released by photons at 350 nm. Further experiments were carried out to deliver the chemotherapy drug doxorubicin in a similar manner in vitro. Our results show a net release rate of 65% within 10 min, and the released drug maintained its therapeutic effect. This hydrogel system provides a promising platform for drug delivery in targeted therapy and in biotechnological applications.