Levels of Phytosterol Oxides in Enriched and Nonenriched Spreads: Application of a Thin-Layer Chromatography-Gas Chromatography Methodology
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- 作者：Ana Conchillo ; Luisito Cercaci ; Diana Ansorena ; Maria Teresa Rodriguez-Estrada ; Giovanni Lercker ; and Iciar Astiasará ; n
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2005
- 出版时间：October 5, 2005
- 年：2005
- 卷：53
- 期：20
- 页码：7844 - 7850
- 全文大小：80K
- 年卷期：v.53,no.20(October 5, 2005)
- ISSN：1520-5118
文摘
The content of phytosterol oxidation products (POPs) in enriched and nonenriched commercial spreadswas evaluated by thin-layer chromatography-gas chromatography (TLC-GC). Oxides of 
-sitosterol,campesterol, and stigmasterol were produced by thermo-oxidation (7-hydroxy, 7-keto, and epoxyderivatives) and chemical synthesis (triol derivatives), which were then separated and identified byTLC-GC. Their identification was further confirmed by GC-mass spectrometry (GC-MS). The totalamounts of phytosterols found were 6.07 and 0.33 g/100 g of sample in phytosterol-enriched andnonenriched spread, respectively, whereas the total POPs contents were 45.60 and 13.31 mg/kg ofsample in the enriched and nonenriched products. The main POPs found were the 7-keto derivativesof all phytosterols analyzed; 7-ketositosterol was the most abundant one (14.96 and 5.93 mg/kg ofsample in phytosterol-enriched and nonenriched spread). No -epoxy and triol derivatives weredetected in both types of samples. The enriched spread presented a lower phytosterol oxidation rate(0.07%) than the nonenriched one (0.41%).Keywords: Phytosterol-enriched spread; phytosterols oxidation products; functional foods
-sitosterol,campesterol, and stigmasterol were produced by thermo-oxidation (7-hydroxy, 7-keto, and epoxyderivatives) and chemical synthesis (triol derivatives), which were then separated and identified byTLC-GC. Their identification was further confirmed by GC-mass spectrometry (GC-MS). The totalamounts of phytosterols found were 6.07 and 0.33 g/100 g of sample in phytosterol-enriched andnonenriched spread, respectively, whereas the total POPs contents were 45.60 and 13.31 mg/kg ofsample in the enriched and nonenriched products. The main POPs found were the 7-keto derivativesof all phytosterols analyzed; 7-ketositosterol was the most abundant one (14.96 and 5.93 mg/kg ofsample in phytosterol-enriched and nonenriched spread). No -epoxy and triol derivatives weredetected in both types of samples. The enriched spread presented a lower phytosterol oxidation rate(0.07%) than the nonenriched one (0.41%).Keywords: Phytosterol-enriched spread; phytosterols oxidation products; functional foods