文摘
Irritable Bowel Syndrome (IBS) is a common multifactorial intestinal disorder for which the aetiologyremains largely undefined. Here, we have used a Trichinella spiralis (T. spiralis)-induced model of post-infective IBS, and the effects of probiotic bacteria on gut dysfunction have been investigated using ametabonomic strategy. A total of 44 mice were divided into four groups: an uninfected control groupand three T. spiralis-infected groups, one as infected control and the two other groups subsequentlytreated with either Lactobacillus paracasei (L. paracasei) NCC2461 in spent culture medium (SCM) orwith L. paracasei-free SCM. Plasma, jejunal wall and longitudinal myenteric muscle samples werecollected at day 21 post-infection. An NMR-based metabonomic approach characterized that the plasmametabolic profile of T. spiralis-infected mice showed an increased energy metabolism (lactate, citrate,alanine), fat mobilization (acetoacetate, 3-D-hydroxybutyrate, lipoproteins) and a disruption of aminoacid metabolism due to increased protein breakdown, which were related to the intestinal hypercontractility. Increased levels of taurine, creatine and glycerophosphorylcholine in the jejunal muscles wereassociated with the muscular hypertrophy and disrupted jejunal functions. L. paracasei treatmentnormalized the muscular activity and the disturbed energy metabolism as evidenced by decreasedglycogenesis and elevated lipid breakdown in comparison with untreated T. spiralis-infected mice.Changes in the levels of plasma metabolites (glutamine, lysine, methionine) that might relate to amodulation of immunological responses were also observed in the presence of the probiotic treatment.The work presented here suggests that probiotics may be beneficial in patients with IBS.